Castillo, Paulo Eugenio B. (2014) Isolation and characterization of bacteriophage for Pseudomonas aeruginosa. Undergraduate thesis, De La Salle University-Dasmarinas.

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Abstract

This undergraduate research was conducted to isolate and characterize the bacteriophage for Pseudomonas aeruginosa. Wastewater was obtained from the sewage system of De La Salle University Medical Center and was enriched with decastrength phage broth containing an overnight broth culture of P. aeruginosa. The broth was membrane filtered after 24 hours and the filtrate was tested on a newly swabbed lawn of P. aeruginosa, in which after another 24 hours showed clearing, confirming its plaque-forming properties against the bacteria. The isolates were then sent to the Research Institute of Tropical Medicine for electron micrographs that was used for characterization and morphology. Based on the gathered data, findings revealed that the isolated bacteriophage that infected P. aeruginosa is the Φ6 bacteriophage, a tailless bacteriophage infecting Pseudomonas species in icosahedrons and dodecahedron variants. This study concluded that the isolated bacteriophage was capable of inhibiting the growth of P. aeruginosa.

Item Type:	Thesis (Undergraduate)
Additional Information:	BIO 1087 2009
Subjects:	Q Science > QH Natural history > QH301 Biology Q Science > QR Microbiology
Users:	College of Science and Computer Studies > Biological Science
Depositing User:	Users 4 not found.
Date Deposited:	04 Dec 2015 02:35
Last Modified:	21 Apr 2022 03:17
URI:	https://thesis.dlsud.edu.ph/id/eprint/254

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