Al Dhaheri, Sara Ali A. (2010) The Effects of the toxin of Echinothrix calamaris (Haptin urchin) in the reflex of Mus musculus (albino mice). Undergraduate thesis, De La Salle University - Dasmariñas.
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Abstract
The study is about the effects of toxin of Echinothrix calamaris to the reflex of Mus musculus. Echinothrix calamaris is a kind of sea urchin that belongs to family of Diadematidae. Sea urchins are known to possess a toxin. Echinothrix calamaris is very distinctive because it is the only sea urchin that has a banded spine. Echinothrix calamaris has toxin that has enzyme that can have an effect in the muscle contraction. Echinothrix calamaris toxin can also be poisonous enough to kill an organism. LD50 is the accurate concentration of toxin that can kill the test organism. Dilution of the toxin is also significant to secure the correct concentration of the toxin in order to have an interaction to the other enzyme of the body. The Range Finding Test is obtained by arriving at different concentrations and this concentrations are determined by Logarithmic method at 0.03 intervals. There are four test organism in each treatments in Range Finding Test. In the Range Finding Test, the concentration of the toxin that is the LD50 is 0.75cc, meaning it is 75% of the toxin. The Eye Boggle Test, Flipping Test and Tail Pricking Test are used to test the reflex of the Albino mice. The Eye Boggle test is for consciousness. The Flipping Test is a test for balance. The Tail Pricking Test is a test for pain reception. The null hypothesis was accepted meaning there is significant difference in the effects of the dose of the toxin of Echinothrix calamaris to the reflex of Mus musculus. It was concluded that the toxin has an effect in the muscle contraction, but not in the consciousness which falls under the nervous system. The enzymes present are the Two D-galactose binding lectin and heparin binding lectin. Also Contractin A is present.
Item Type: | Thesis (Undergraduate) |
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Additional Information: | CALL NUMBER : BIO 1117 2010 |
Keywords: | Toxin. |
Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QL Zoology |
Users: | College of Science and Computer Studies > Biological Science |
Depositing User: | Ivyjoy Viray |
Date Deposited: | 28 Nov 2015 00:58 |
Last Modified: | 10 Oct 2024 08:33 |
URI: | https://thesis.dlsud.edu.ph/id/eprint/917 |
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