Gnilo, John Royce D. and Sumague, Mark C. (2013) Prevalence of multi-drug resistant Salmonella typhi in street foods in Dasmariñas City, Cavite. Undergraduate thesis, De La Salle University - Dasmariñas.

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Abstract

The aim of this study was to determine the occurrence of multidrugresistant Salmonella Typhi in street foods in Dasmariñas City, Cavite. A total of 31samples of street food samples were purchased at random from street foods stalls in Dasmariñas, Cavite, newly grilled or cooked. Each of the food samples was blended and separated according to its kind. After enrichment, it was plated into Salmonella -Shigella Agar and incubated at 37ºC for 24 hrs. Black colonies were maintained in brain heart infusion broth (BHI). Total genomic DNA was isolated using Wizard ® Genomic DNA Purification Kit (Promega 2010). The isolated Salmonella were confirmed using ompC gene. A fragment of 204 base pairs (bp) of the ompC gene was selected. Detected ompC gene was amplified and runned in gel electrophoresis. Lastly, antimicrobial susceptibility of Salmonella spp. was also determined. Twenty one out of thirty one or sixty eight percent was the prevalence of Salmonella spp. in the available street foods and there were no Salmonella Typhi present in available street foods.

Item Type:	Thesis (Undergraduate)
Additional Information:	CALL NUMBER : BIO 1243 2013
Keywords:	Street foods.
Subjects:	Q Science > QH Natural history > QH301 Biology Q Science > QR Microbiology
Users:	College of Science and Computer Studies > Biological Science
Depositing User:	Ivyjoy Viray
Date Deposited:	25 Nov 2015 01:48
Last Modified:	25 Nov 2015 01:48
URI:	https://thesis.dlsud.edu.ph/id/eprint/767

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