Inhibitory effect of tea tree oil and virgin coconut oil on multidrug-resistant pseudomonas aeruginosa.

Mancia, Chester A. and Viray, Danielle (2010) Inhibitory effect of tea tree oil and virgin coconut oil on multidrug-resistant pseudomonas aeruginosa. Undergraduate thesis, De La Salle University - Dasmariñas.

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Abstract

Pseudomonas aeruginosa is one of the leading causes of hospitalassociated and ventilator-acquired pneumonia and their growing resistance to large variety of antibiotics makes it harder to be treated. Multi-drug resistant Pseudomonas aeruginosa (MDRPA) infections limit treatment options and have forced clinicians to reconsider the use of older and more toxic agents. This study used the essential oils namely Tea Tree Oil (TTO) and Virgin Coconut Oil (VCO) as alternative treatments againts Multi-drug resistant Pseudomonas aeruginosa. Pseudomonas aeruginosa was subjected to four drugs (Ceftazidime, Ciprofloxacin, Imipenem and Gentamicin) for a total of seven days to induce multi-drug resistance. For the monotherapeutic treatment, the suspected MDRPA was seeded on Mueller-Hinton agar (MHA) with disks impregnated with VCO (T1), TTO (T2) and Carbenicillin (T0) as treatment and incubated at 37°C for 24 hrs. Monotherapy was also performed together with the combined therapy for the mixed culture assay to asses the density of the microorganism. Serial dilution up to 10-7 was prepared using 1mL inoculum and 9mL dilution blank. Dilutions of 10-5 to 10-7 were spread plated into Cetrimide agar and incubated at 37°C gor 24 hrs. The density of multi-drug resistant Pseudomonas aeruginosa was measured every two days for ten days. In the monotherapy treatment Tea Tree Oil and Virgin Coconut Oil showed a zone of inhibition of 8 millimeter and 6 mm respectively. This result showed that the MDRPA was resistant to the essential oils. The result in mixed culture assay for monotherapy of VCO decreased from 6.40x1009 cfu/ml to 1.33x1008, TTO decreased from 5.10x1009 cfu/ml to 1.00x1008 and combined therapy decreased from 5.97x1009 cfu/ml to 7.00x1008 cfu/ml it showed a decline in the number of colony forming units but did not show any significant difference (p > 0.05). The two essential oils were not effective in inhibiting the growth of multidrug resistant Pseudomonas aeruginosa.

Item Type: Thesis (Undergraduate)
Additional Information: CALL NUMBER : BIO 1119 2010
Keywords: Inhibitory effect ; Pseudomonas aeruginosa
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QK Botany
R Medicine > RM Therapeutics. Pharmacology
Users: College of Science and Computer Studies > Biological Science
Depositing User: Ivyjoy Viray
Date Deposited: 25 Nov 2015 08:57
Last Modified: 25 Nov 2015 08:57
URI: http://thesis.dlsud.edu.ph/id/eprint/915

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