Screening and identification of oil-degrading bacteria isolated fromoil-contaminated soils of Rosario, Cavite.

Eleserio, Stephen Y. and Francisco, Alvin John P. (2013) Screening and identification of oil-degrading bacteria isolated fromoil-contaminated soils of Rosario, Cavite. Undergraduate thesis, De La Salle University - Dasmariñas.

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Abstract

With the increasing cases of oil spills in the Philippines, bioremediation becomes a non-invasive and cost-effective solution. This study aimed to isolate, screen and identify bacteria capable of oil-degradation and treating oil-contaminated wastewater. Bacteria were enriched and isolated from oil-contaminated soils of Rosario, Cavite and subjected to microscopic examination after Gram-staining method to determine their morphological characteristics. Isolates were screened for their capability to degrade crude-oil in simulated wastewater. Five set-ups were prepared comprising the control and treatments. Their efficiency for crude oil biodegradation was demonstrated by determining the amount of oil concentration reduced, pH level and biochemical oxygen demand (mg/L) in wastewater samples. Reduced crude oil concentration was defined through oil and grease analysis by Partition-gravimetric method. Afterwards, bacteria were identified using API 20E (BioMérieux®) and BBL Crystal Non-fermenter/enteric (BBL Crystal™) identification kits. The results showed that isolates labeled as oil-degrading 1 (OD1) and oil-degrading 3 (OD3) were found to be both gram-negative rod bacteria with sole arrangement while oil-degrading 2 (OD2) be gram-positive rod bacterium. All isolates were capable for degrading reducing the crude oil concentration before and after incubation (p = 0.0001). OD1, OD2 and OD3 were identified as Proteus vulgaris, Bacillus subtilis and Pseudomonas aeruginosa, respectively. Application of these isolates in bioremediation process can be cost-effective measures to the oil clean up.

Item Type: Thesis (Undergraduate)
Additional Information: CALL NUMBER : BIO 1234 2013
Keywords: Oil-degradation
Subjects: Q Science > QH Natural history > QH301 Biology
Users: College of Science and Computer Studies > Biological Science
Depositing User: Ivyjoy Viray
Date Deposited: 25 Nov 2015 05:03
Last Modified: 25 Nov 2015 05:03
URI: http://thesis.dlsud.edu.ph/id/eprint/803

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