Toxicity screening of alkaloids from tabernaemontana pandacaqui POIR (Pandakaki-puti) and voacanga globosa (Blanco) MERR.(Bayag-Usa) leaf extracts using brine shrimp assay.

Andaya, Genesis Faye and Hulipas, Gladys Dianne (2012) Toxicity screening of alkaloids from tabernaemontana pandacaqui POIR (Pandakaki-puti) and voacanga globosa (Blanco) MERR.(Bayag-Usa) leaf extracts using brine shrimp assay. Undergraduate thesis, De La Salle University-Dasmarinas.

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Abstract

The alkaloids from Tabernaemontana pandacaqui and Voacanga globosa leaves were selectively extracted, quantified, and tested for their cytotoxic activity using the Brine Shrimp Lethality Assay (BSLA). The extraction procedures included methanol extraction, Mayer’s reagent precipitation, and chemically active solvent extraction. The alkaloid content of the extracts were quantified as milligrams of Vincristine Sulfate Equivalent (mg VSE) using Bromocresol Green Assay. The results showed that the average alkaloid content of T. pandacaqui and V. globosa leaves are 46.7 mg VSE and 105.3 mg VSE which translated to percent yield of 0.234% and 0.527% respectively. These values may seem very low but taking into context that the standard alkaloid, vincristine from Cantarantus roseus, has a percent yield between 0.20-1.0% - the obtained percent yield is comparatively within the normal range of alkaloid yield from plants of Apocynaceae family. BSLA studies revealed that the LC50 values of T. pandacaqui and V. globosa leaves are of 167 ppm and 40.8 ppm respectively. These values are higher than the LC50of vincristine sulfate at 5.03 ppm, which means that they are less cytotoxic than the standard used. However, they are still well within the defined threshold of cytotoxic potency at <200 ppm. This warrants the further investigation of their alkaloid content for their cytotoxic action which may prove useful in cancer chemotherapy.

Item Type: Thesis (Undergraduate)
Additional Information: BIO 1176 2012
Keywords: Tabernaemontana ; Plant extracts ; Toxicity.
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QK Botany
Users: College of Science and Computer Studies > Biological Science
Depositing User: Ivyjoy Viray
Date Deposited: 12 Nov 2015 05:04
Last Modified: 12 Nov 2015 05:04
URI: http://thesis.dlsud.edu.ph/id/eprint/503

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