Magpantay, Marielle A. and Sembrano, Nicole Marie D. (2012) Ineffectivity potential of induced viable but nonculturable overt pathogenic enterobacters. Undergraduate thesis, De La Salle University-Dasmariñas.

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Abstract

The occurrence of waterborne diseases is increasing throughout the Philippines particularly in Cavite province. Enteric bacteria are responsible for food poisoning and high rate of diarrhea cases. Some of the enteric bacteria enter viable but nonculturable state (VBNC) which can be a public health problem. The study investigated the infectivity potential of induced viable but nonculturable overt pathogenic enterobacters, Eshcerchia coli, Salmonella sp. and Shigella sp. Enteric bacteria were isolated from the ground water of Malagasang, Anabu Kostal, Imus-Cavite. Identification of the isolates was done by streak plate method using Eosin Methylene Blue Agar and Salmonella Shigella Agar and confirmed by API-20E kit. The identified isolated enteric bacteria were subjected to induction of viable but nonculturable state through nutrient deprivation and or heat stress. The results showed that among the 9 water samples, 7 were positive to E.coli, 5 for Salmonella sp. and 2 for Shigella sp. The isolated Escherichia coli and Salmonella sp. upon subjection to nutrient deprivation and or heat stress were able to enter VBNC state for 15 days. However, the infectivity potential of the induced VBNC bacteria was not observed in animal model (albino mice). No observable symptoms relating to diarrhea were observed such as watery and bloody diarrhea for 2 days. No immediate deaths were recorded. With these results, it can be concluded that the ground water samples of poor quality and the isolated Escherichia coli and Salmonella sp. that entered VBNC were not infective. The results indicate a need for the province of Cavite to take proper measure in safeguarding public health.

Item Type:	Thesis (Undergraduate)
Additional Information:	BIO 1185 2012
Keywords:	Enterobacters
Subjects:	Q Science > QH Natural history > QH301 Biology Q Science > QR Microbiology
Users:	College of Science and Computer Studies > Biological Science
Depositing User:	Ivyjoy Viray
Date Deposited:	12 Nov 2015 04:47
Last Modified:	28 Jun 2021 05:43
URI:	https://thesis.dlsud.edu.ph/id/eprint/491

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