Detection of salmonella typhi in farmed shellfishes using conventional culture method and polymerase chain reaction, and their antibiotic resistance.

Istino, Maria Klenna Rose A. and Kapulong, Anna Marie Josiefine C. (2013) Detection of salmonella typhi in farmed shellfishes using conventional culture method and polymerase chain reaction, and their antibiotic resistance. Other thesis, De La Salle University-Dasmariñas.

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Abstract

A study was performed in order to determine the prevalence of Salmonella Typhi and relate its presence in the type of farmed shellfishes (mussels and oysters) and season (hot dry, wet, cool dry) in Cavite City. A total of 30 samples (15 from mussels and 15 from shellfish) were collected from Cavite City during April, June and November. The physico chemical characteristics of water such as salinity, conductivity, temperature, dissolved oxygen and pH were also obtained. When the conventional culture method was performed, a 100% prevalence of Salmonella spp. was obtained from all the samples collected in different seasons. Using the PCR method, (ompC and viaB), a 90% prevalence of Salmonella spp. was detected in mussel isolates during the hot dry and wet season but not in dry season. On the other hand, Salmonella spp. isolated from oysters were prevalent during the hot dry and cool dry season and 70% prevalent in wet season. However, no Salmonella Typhi was observed in all of the samples. In determining the association between Salmonella spp. and predictor variables, the type of shellfish (P =0.04) and season (P =0.05) were found to be good predictors. Oysters obtained during hot dry season yield a favorable growth of Salmonella spp .In the antimicrobial susceptibility testing, 37% of bacterial isolates from the shellfishes were considered as multidrug resistant bacteria since it has shown resistance to the all of the antibiotics (ampicillin, chloramphenicol, streptomycin, and tetracycline).

Item Type: Thesis (Other)
Additional Information: BIO 1216 2013
Keywords: Salmonella typhi ; Polymerase
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QR Microbiology
S Agriculture > SH Aquaculture. Fisheries. Angling
Users: College of Science and Computer Studies > Biological Science
Depositing User: Ivyjoy Viray
Date Deposited: 11 Nov 2015 05:46
Last Modified: 11 Nov 2015 05:46
URI: http://thesis.dlsud.edu.ph/id/eprint/432

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