Prevalence of shiga-toxin producing Escherichia coli in beef sold in Dasmariñas City markets.

Castro, Arizaldo E. and Urquia, Thessa Marie L. (2013) Prevalence of shiga-toxin producing Escherichia coli in beef sold in Dasmariñas City markets. Undergraduate thesis, De La Salle University-Dasmariñas.

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Abstract

This study was undertaken to establish the prevalence of Shiga-toxin producing Escherichia coli (STEC) in beef sold in three major public markets of Dasmariñas city and associate the presence with the type of meat sourced and the season of collection. Thirty (30) beef samples (15 ground beef and 15 beef patties) collected during the dry season (April and May) and wet season (June, August, October) were processed via bacterial enrichment with Lactose broth and plated onto Sorbitol MacConkey‟s Agar for isolation of pathogenic E.coli colonies. Subsequent DNA extraction was carried out to procure the whole genomic DNA of the E.coli isolates. Extracted DNAs were subjected for Polymerase Chain Reaction Assay of two STEC indicative genes namely stx1 which codes for Shiga-toxin type I and rfbO157 which is a marker of the notorious Enterohemorrhagic E.coli type species, E.coli O157. Analyzed gel slabs showed 3 ground beef samples to be positive for stx1. Also, 2 from these stx1 positive samples yielded positive with rfbO157. Prevalence of STEC was calculated to be at 10%. Analysis using multiple logistic regression showed that season and the type of meat are not associated with the presence of STEC suggesting that they are not good predictors of the possibility of detecting this E.coli pathotype (p<0.05). In addition, since non-O157 STEC and E.coli O157 are identified, they can be considered as possible etiologic agents of bloody diarrhea cases in the locality of Cavite that may result to Hemolytic Uremic Syndrome in children and Thrombotic Thrombocytopenic Purpura in adults. The establishment of the presence of these infamous pathogenic bacteria in the province posts a major health threat to the public.

Item Type: Thesis (Undergraduate)
Additional Information: BIO 1226 2013
Keywords: 1. Shiga-toxin.
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Q Science > QR Microbiology
Users: College of Science and Computer Studies > Biological Science
Depositing User: Ivyjoy Viray
Date Deposited: 10 Nov 2015 09:07
Last Modified: 11 Nov 2015 02:44
URI: http://thesis.dlsud.edu.ph/id/eprint/420

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