Phylogenetic analysis of Alocasia zebrina complex (araceae)inferred from chloroplast DNA.

Kaur, Kamaljit and Miranda, Therese Margarett J. (2013) Phylogenetic analysis of Alocasia zebrina complex (araceae)inferred from chloroplast DNA. Other thesis, De La Salle University-Dasmariñas.

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Abstract

Previous studies on Alocasia zebrina complex revealed that the taxa belonging to this group have different morphological characteristics and a subject of further investigation. The study has aimed to do molecular analyses in order to resolve the said taxonomic dilemma. Total genomic DNA of the three samples were extracted, isolated amplified and purified. The primers amplified for each of the samples were at regions of trnL-F and trnK. Alignment of DNA sequences were done through the use of CodonCode Aligner v.4.0 and for the construction of phylogenetic tree MEGA v.5.0 was used. There are 956 characters obtained for the primer trnL-F while 842 for the matK. Through the obtained base frequencies, chi-square of homogeneity and distance matrix, it was found out that the three species are exactly similar with each other. “P” values of 0.99 and 1.00 were obtained for the primers trnL-F and trnK respectively, suggests to have accepted the null hypothesis on the species’ resemblance. In addition to that the constructed phylogenetic tree has presented the three Alocasia taxa are monophyletic and has obtained a bootstrap percentage greater that 50%, specifically 63% and 79% for the aforementioned order of primers. The values only suggest that there is a greater plausibility in terms of their uniformity. The results proved that the taxa under the Alocasia zebrina complex are similar and there is no significant difference within their genomic sequences; hence, they belong to the Alocasia zebrina.

Item Type: Thesis (Other)
Additional Information: BIO 1230 2013
Keywords: 1. Alocasia zebrina complex. 2. Chloroplast DNA.
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Users: College of Science and Computer Studies > Biological Science
Depositing User: Ivyjoy Viray
Date Deposited: 10 Nov 2015 08:34
Last Modified: 11 Nov 2015 02:04
URI: http://thesis.dlsud.edu.ph/id/eprint/416

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