Anti-tumor potential of banana (Musa sapentum) leaf ointment of male albino mice (Mus musculus) exposed to ultra violet light.

Oliva, Mark Moses D. and Dimaano, Peter Bob Z. (2010) Anti-tumor potential of banana (Musa sapentum) leaf ointment of male albino mice (Mus musculus) exposed to ultra violet light. Other thesis, De La Salle University-Dasmariñas.

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Abstract

Due to the increasing case of human cancer in the Philippines there had been researches that investigated the anti-tumor potential of various different types of plants. This study was conducted to explore on the anti-tumor potential of banana leaf in ointment preparation. The objectives of the study is to determine the anti-tumor potential of banana leaf ointment in different concentrations on Albino mice exposed to UV light. The second objective of the study is to determine if there is a significant difference of skin tumor on male Albino mice exposed to UV light administered with various concentrations of banana leaf extract in terms of incidence and tumor size. This experimental study is about the anti-tumor potential of banana leaf ointment on the test subject. Approximately 6-7 weeks old mice were used. The aim of the study was to explore the potential of banana leaf in the prevention of the occurrence of induced skin cancer on male Albino mice. However, the study did not focus on the other chemical components of banana leaf other than polyphenols and anti-oxidants only that were responsible for such prevention. The parameter focused on the area of tumor growth on the skin of Albino mice. Formations of other abnormalities induced during the experiment that are located on other parts aside from the skin were not explored. The method of the research performed was experimental using a Randomized Complete Block Design (RCBD). In the preparation of the banana leaf ointment extract the researchers prepared 100 ml vegetable oil and 30 ml of banana leaf, was fried and strained after the leaves turned to golden brown. A solution of 20 ml grated candle wax was added while the extraction was hot and let cool that made a 30% concentration. The same procedure was done to the 60% and 90% concentration. Before the exposure of the subject organism to UV light, the extract with specific concentration was applied topically to the shaved dorsal portion of the Albino mice. The same application was done after the exposure to the UV light. In the inducement of tumor, the shaved dorsal part of the mice was exposed daily in an interval of 6 hours from 8 am-2 pm. This routine lasted 6 weeks prior to the experiment. The diagnosis and confirmation of skin tumor growth was done by a licensed Pathologist. In the evaluation of tumor growth the study used the hemiellipsoid model in measuring the skin tumor area. A descriptive analysis was performed to determine the anti-tumor potential of banana leaf ointment extract concentration on the skin of male Albino mice exposed to UV light. The incidence of tumor growth per week is significant in performing the descriptive analysis. The test that was used was the oneway Analysis of Variance (ANOVA). This test is used to determine the significant difference on the incidence of skin tumor formation in albino mice applied with various banana leaf extract concentration. In the histopathological analysis of the tumor growth, the licensed pathologist diagnosed the tumor as a Seborrheic Keratosis. The incidence of tumor formed was observed at the fourth week of exposure to UV light in treatment T0 which was the control group and treatment T1 which had 30% concentration. The succeeding weeks showed the formation of tumor growth in all the treatments. The study compared the different groups of concentrations administered to the skin of Albino mice. The study used One-way Analysis of variance(ANOVA) to show the differences of variance between the groups being compared. Since P value is <Fcrit value at (0.5), there is no significant difference on the incidence of skin tumor growth of albino mice administered with various concentration of extract. The results of the study showed that various components of banana leaf had no effect in inhibiting the incidence of skin tumor in albino mice exposed to UV light. The oneway analysis of variance test also showed that there was no significant difference on the incidence of skin tumor in albino mice administered with various concentration of banana leaf extract.

Item Type: Thesis (Other)
Additional Information: BIO 1261 2010
Keywords: 1. Banana (Musa sapentum). 2. Male albino mice (Mus musculus).
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QK Botany
Q Science > QL Zoology
Users: College of Science and Computer Studies > Biological Science
Depositing User: Ivyjoy Viray
Date Deposited: 10 Nov 2015 05:45
Last Modified: 28 May 2020 09:49
URI: http://thesis.dlsud.edu.ph/id/eprint/401

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