A preliminary study on the callus formation of Strongylodon macrobotrys (Jade vine) through tissue culture.

Sierra, Rei Fabbie F. and Victoria, Sharlene Mae D. (2009) A preliminary study on the callus formation of Strongylodon macrobotrys (Jade vine) through tissue culture. Undergraduate thesis, De La Salle University-Dasmarinas.

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Abstract

The preliminary study on the callus formation of Strongylodon macrobotrys (Jade Vine) through tissue culture aims to induce callus on the Adopted Murashige and Skoog medium and compare the effects of amounts of α- Naphthalene Acetic Acid with Hormex, a commercial plant hormone. The researchers managed to inoculate the internodes of Jade Vine in the adopted media used in tissue culture. On the first trial, the internodes used as explants on the media bottles showed no response to the adopted media in the 1st week of inoculation and on the following week, the discoloration or darkening of the internodes happened, suggesting the presence of cellular decomposition on the explants. Based on the above results, the researchers added more hormones on the second trial. This time, the internodes showed no response for two weeks after inoculation and started to darkened on the third week which is the expected callus induction time. The results suggested that exploring other suitable media, increasing the volume of the applied hormones and determining the amount of phenolic compounds present in the tissue culture should be done to ensure the callus formation of Jade Vine.

Item Type: Thesis (Undergraduate)
Additional Information: BIO 1052 2009
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QK Botany
Users: College of Science and Computer Studies > Biological Science
Depositing User: Ms. Bibiana Alcantara
Date Deposited: 07 Dec 2015 01:24
Last Modified: 16 Oct 2020 06:49
URI: http://thesis.dlsud.edu.ph/id/eprint/275

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